This
server calculates a consensus melting temperature (Tm) for
any given short DNA sequence (16-30 nts) using a merged method
that is based on three different thermodynamic tables. The
consensus Tm value is a robust and accurate estimation of
melting temperature for short DNA sequences of practical application
in molecular biology. Accuracy benchmarks using all experimental
data available indicate that the consensus Tm prediction errors
will be within 5 ºC from the experimental value in 89%
of the cases. |