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 Melting
temperature calculation servers |
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| Name |
Type |
Method(s) |
Warning |
Restricted
Input |
Reference |
| Oligo Tm predictor |
Server |
All97,
San98 |
Yes |
No |
No |
| Melting |
Server |
Bre86,
San96, Sug96, All97 |
No |
No |
Yes(1) |
| Melting Temperature Prediction |
Server |
Bas |
No |
No |
No |
| Oligo
Analyzer |
Server |
All97 |
Partial |
No |
No |
| DAN |
Server |
Bre86 |
No |
No |
No |
| Oligonucleotide Properties Calculator |
Server |
Sug96 |
Partial |
No |
No |
| Tm
Determination |
Server |
Bre86 |
No |
No |
No |
| BioMath Tm Calculations for Oligos |
Server |
Bas,
Sal, San98 |
Yes |
No |
No |
| Melting temperature for DNA oligos |
Server |
San98 |
No |
No |
No |
| Oligonucleotide Tm Calculations |
Server |
Bas,
Sal, Bre86 |
Partial |
No |
No |
| Proligo: oligo calculation |
Server |
Bas,
Bre86 |
Partial |
No |
No |
| QuickChange primer Tm calculator |
Server |
Bas |
No |
No |
No |
| Tm Calculator |
Server |
No
info |
No |
No |
No |
| Oligo Calculator |
Server |
Bas |
No |
No |
No |
| TmCalc |
Software |
Bas |
N/A |
N/A |
N/A |
| Tm-Calculator |
Server |
N/A |
No |
No |
No |
| Oligo Melting Temperature |
Server |
Bre86,
San98 |
No |
No |
No |
|
(1)
Bioinformatics 17, 1226-27, (2001).
|
Abbreviations
and references
>
Bas:
Basic Tm calculation: Marmur and Doty (1962) J. Mol. Biol.
5, 109-118.
> Sal: Salt
adjusted Tm calculation: Howley et al., (1979) J. Biol.
Chem. 254, 4876-4883.
> Bre86:
Tm calcularion based on Nearest-neighbor thermodynamics: Breslauer
et al. (1986) PNAS 83, 3746-3750.
> San96:
Tm calcularion based on Nearest-neighbor thermodynamics: SantaLucia
et al. (1996) Biochemistry 35, 3555-3562.
> Sug96:
Tm calcularion based on Nearest-neighbor thermodynamics: Sugimoto
et al. (1996) Nucleic Acids Res. 24 , 4501-4505.
> San98:
Tm calcularion based on Nearest-neighbor thermodynamics: SantaLucia
(1998) PNAS 95, 1460-1465.
> All97:
Tm calcularion based on Nearest-neighbor thermodynamics: Allawi
and SantaLucia (1997) Biochemistry 36, 10581-10594.
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