SUPPLEMENTAL MATERIAL FOR

 

Identification of novel transcripts with differential dorso-ventral expression

in Xenopus gastrula using serial analysis of gene expression

Fernando Faunes, Natalia Sánchez, Javier Castellanos, Ismael Vergara, Francisco Melo and Juan Larraín.

 

 

LARGE DATA FILES NOT AVAILABLE AT THE JOURNAL WEB SITE (GENOME BIOLOGY)

WWW LINK

DESCRIPTION

Experimental tags in genome

A text file containing a tabulated list of experimental SAGE tags from our experiment, mapped to the genome, along with several calculated information (see Header_Experimental_tags_in_genome.doc for a description of the columns in this table).

Virtual tags in genome

A text file containing a tabulated list of all potential virtual NlaIII-BsmFI tags in the Xenopus genome, along with several calculated information (see Header_Virtual_tags_in_genome.doc for a description of the columns in this table).

Ensembl sequences

A text file in FASTA format containing the 28,657 RNAs from Ensemble.

NCBI sequences

A text file in FASTA format containing the 7,976 RNAs from NCBI.

Unigene sequences

A text file in FASTA format containing the 42,654 EST clusters from Unigene.

Gurdon sequences

A text file in FASTA format containing the 41,921 EST clusters from the Gurdon Institute.

 

DATA FILES AVAILABLE AT THE JOURNAL WEB SITE (GENOME BIOLOGY)

WWW LINK

DESCRIPTION

Additional_data_file_description.txt

This file contains a consolidated and sumarized description of the contents of each additional file listed below.

Additional_data_file_01.pdf

Includes Supplementary Tables 1-4 and their legends. Supplementary Table 1 contains the tags derived from known genes with differential expression along the dorso-ventral axis and their frequency of occurrence in our SAGE libraries. Supplementary Table 2 contains the tag-mapping to transcripts for the 125 tags with significant difference of frequency of occurrence between both libraries. Supplementary Table 3 contains reverse SAGE sequences. Supplementary Table 4 contains the sequences of the primers and information about the probes used in this study.

Additional_data_file_02.xls

Contains a table with the list of 180 experimental tags with p-values < 0.05 (obtained with the 3 statistical tests), information about known genes and count fold-ratios.

Additional_data_file_03.pdf

Includes Supplementary Figure S1.

Additional_data_file_04.xls

Contains the complete list of experimental tags with their frequencies of occurrence in the SAGE libraries, normalized count ratio and p-values from three different statistical tests.

Additional_data_file_05.xls

Contains 15-nts-long experimental tag sequences, their frequencies in the SAGE libraries, count ratios, p-values and their corresponding sequences of 14-nts tags.

Additional_data_file_06.txt

Contains the list of experimental tags matching to the genome and to the Ensembl transcripts in positions 1, 2 or polyA-next.

Additional_data_file_07.txt

Contains the list of experimental tags matching to the genome, to the NCBI, Unigenes and Gurdon transcript databases in positions 1, 2 or polyA-next (only those not present in Ensembl).

Additional_data_file_08.txt

Contains the list of experimental tags matching to the genome but without reliable matches to the transcript databases.

Additional_data_file_09.txt

Contains the list of experimental tags matching to the genome but without any match to the transcript databases.

Additional_data_file_10.txt

Contains the list of experimental tags matching to the genome and their frequencies of occurrence in the transcript databases used in this study.

Additional_data_file_11.xls

Tag-mapping of experimental tags to the Ensembl transcript database.

Additional_data_file_12.xls

Tag-mapping of experimental tags to the NCBI transcript database.

Additional_data_file_13.xls

Tag-mapping of experimental tags to the Unigene transcript database.

Additional_data_file_14.xls

Tag-mapping of experimental tags to the Gurdon transcript database.